Library Sample Preparation

  • Prepare a total amount of 400 fmol in 20 μL of nuclease-free water or low TE buffer (this translates to approximately 260 ng for a 1 kb amplicon).
  • Submit samples in 96-well PCR plates or in tube strips with the number indicating the sample number of each strip.
  • For these samples we do recommend Fluorometric Quantification (e.g. Qubit) if possible.