Amplicon Sample Preparation

  • Prepare samples at 50 ng/μL in 10 μL of nuclease-free water or low TE buffer.
  • Submit samples in 96-well PCR plates or in tube strips with the number indicating the sample number of each strip.
  • The minimum size of your submitted PCR product should be 700 bp.
  • We do not routinely recommend Fluorometric Quantification (e.g. Qubit) because this is impractical in most settings. This does mean that when you start using our service your samples might not sequence well at what you measure to be 50 ng/μL, however, by tuning the concentration you will be able to determine a concentration that works for your setup.